Expression vector PET 28+
Desired gene Lipase
Thanks Dr. Vijay
You can use any of the Ni-NTA agarose or Cu-NTA agarose or Co-NTA agarose matrix for his tag affinity chromatography, supplied by a number of companies.
As I am working on various chemical as well as biological synthesized molecules (Bacterial enzymes, plant or fern based compounds). So I want to know the exact receptor on fungus or bacteria...
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Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
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03 March 2021 5,360 2 View
Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...
26 February 2021 3,738 3 View
In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...
26 February 2021 3,806 2 View
Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!
25 February 2021 3,092 2 View
I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...
25 February 2021 8,039 1 View
I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...
25 February 2021 2,388 3 View
I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...
24 February 2021 1,534 2 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...
22 February 2021 1,789 3 View