Hello,
We are planing to use oil red 0 (OR0) in terms of showing which part of skin our microemulsions (ME) and ME based Gel formulations are being trapped by using laser scanning confocal microscope. Is there anybody can help us finding a protocol for this procedure?
Also, do you think it would have disadvantages using previously removed rat skin for this procedure as well?
Thank you very much in advance.
The AFIP Manuel can help you out. Remember that you can not fix your tissues. The way is to do a frozen section on your skins. Once you get a good section, immediately do the staining. There are some good commercial kits that you can purchase.
Gogle the stain,then goggle the kits.
Let me know how your stains come out.
Cynthia Haynes-James
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