Dear Colleagues, with raise in the price of our usual tetracycline-free FBS and given failing of the most recently ofered alternative, I'm asking if you have any advice on a brand offering tetratcycline-free (or very low tetracycline:
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
How do mathematicians recognize the article entitled "On the Nature of Some Euler's Double Equations Equivalent to Fermat's Last Theorem", published in Mathematics, Volume 10, Issue 23 (December-1...
09 May 2024 3,583 6 View
The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).
17 March 2024 4,300 2 View
I need to choose one of these software, any insights or suggestions on which one is the most user friendly and effective. I am towards Jade at the moment, but the cost is substantially higher than...
01 February 2024 5,009 0 View
I am trying to develop Curcumin bioconjugates by esterifying -OH moieties of Curcumin aromatic rings. By performing UV spectrophotometric analysis of the conjugate (red curve) in a mixture...
01 August 2023 5,181 2 View
Very technical question. From my past experience building libraries in S. cerevisiae, after electroporating yeast with a ligation reaction or with plasmid preps, the cells are incubated in liquid...
29 June 2022 1,940 2 View
I use S. cerevisiae for yeast display and I suspect I have a contamination in my cultures but it is not obvious and I don't currently have a microscope in the lab. I grow in minimal media...
11 March 2022 1,605 2 View
I found these specimens in a sample taken from shallow waters, in the Northern Adriatic Sea (Italy). Average width around 16 um and length around 24 um. Asymmetric, with pores scattered on the...
01 September 2021 1,994 2 View
Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...
24 February 2021 1,819 3 View
Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...
24 February 2021 3,096 1 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,747 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,604 7 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,401 4 View
I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?
27 July 2024 5,916 1 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...
24 July 2024 8,245 5 View
I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.
21 July 2024 3,465 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,043 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,928 2 View