Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View