I'm not quite sure that it can help you somehow, but I found and explained (in a very plain language) connections between solving differential equations under null-Laplacian restriction an the message-passing strategy used in the Routing Information Protocol (RIP), which certainly has many analogies to the ACO you are interested in.
It is just a silly guess, but I feel that it may help in your search of a version (or even a new adaptation) of ACO to solve the OTSP.
Unfortunately, it is just a 'home-made' film posted on Youtube, in Portuguese. But I hope it will be useful anyway...
I suggest Ant-Q algorithm for handling NP-hard problem. The Ant-Q algorithm is an agent based algorithm that combines Ant colony based methods with the
reinforcement learning technique.
Personnaly I have used the Ant-Q as hyperheuristic for solving the cutting stock problem and I have reached good results.