Whatman CF 11 cellulose powder (Cat. No. 4021-050) has been used to separate dsRNA elements from ssRNA, ssDNA and dsDNA. Column chromatography under 15% ethanol concentration, CF 11 cellulose specifically binds dsRNA fraction. Now the producer of CF 11, Whatman, does no longer produce it. Does anyone has experience with alternatives? I need it for dsRNA-binding purpose. I look forward to hearing from you...
Hi Subha,
Thanks for your inquiry about the alternative. I was using Whatman cellulose but since last couple of weeks I have been using ''Sigma Type 101 cellulose (catalog #S6790-100g)'' and it seems to me very excellent in performance! I suggest you to try this one and hopefully you will find it commendable.
Best of luck with your dsRNA research!
Hello,
Do you guys think Celite 80-120 mesh could bind dsRNA as cellulose?
Thanks!
Sad! CF11 has been discontinued!! however I use sigma Cellulose fibers, (medium) Cat # c6288 and it works.
The only difference I found is the texture of the two products are different. CF11 seems to absorb the solvent faster than sigma cellulose, Therefore, if your method requires cellulose slurry then you need to mix it with the solvents for longer times.
I do recommend to optimise the parameters including temperature, contact time and equilibrium time as I found slight differences when I started using Sigma cellulose.
I am also at a loss after discovering that CF11 is no longer available, as this is what all the protocols for dsRNA suggest. Rafea, do you use the same amount of # c6288 as you would have for CF11 ? thank you very much for your help.
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