I have to sacrifice pretreated mice models to evaluate the mechanism study. Before killing them I have to collect blood for GTT. I've been informed that I have to give them an interval before killing them (I do not want to make them stressed). Also I need time to collect blood from all of the mice models.
1. I need to know that how long the interval should be before killing them?
2. Should I collect blood from all mice of same group in same day or should I collect a certain number of mice from all of groups?
3. Should the killing order be maintained the same as the blood collection or is random order fine if the interval is about 3-4 days?
I really don't know much about your experiment from the information you've provided however my suggestion would be:
1) Use the interval period used in previous literature, assuming that the method has been done before
2) Depending on your time constraints and how many animals you have, will determine how you collect. I'm assuming you have a lot of animals, so perhaps it is better to take a sample from each group on the same day rather than taking one group at a time. This should help you control for changes across days between groups and perhaps see if there are any changes across time within groups.
3) Not really sure about this one. Again I would refer to previous literature. I would think that after the interval where animals have returned to baseline stress levels, it shouldn't matter which order you kill them.
I Hope this helps you
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