i would recommend you aliquots 10-15 micro liter in each eppendorf tube and store at -20 °C to -70 °C . you don't need to dilute the antibody.

For most antibodies specific recommendations are not available. The data sheet for this polyclonal antibody says: "Supplied as solution form in PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol."

From my point of view, the product should be aliquoted directly without any dilution and stored at - 20°C. Higher dilutions need to be prepared freshly. BTW: Glycerol (or glycerin) is not the same as glycine.

I would recommend the same. Just store the aliquots directly at -20 ° C without any dilution and avoid repeated freeze/thaw cycles. The antibody should be stable for at least 1 year.

i would recommend you aliquots 10-15 micro liter in each eppendorf tube and store at -20 °C to -70 °C . you don't need to dilute the antibody.

For antibody storage in general, make sure your freezer is not a frost-free one. A frost-free freezer will freeze and thaw in cycles to remove any frost build up and this will inactivate your antibodies and defeats the purpose of aliquots. In this particular case, the sample contains 50% glycerol, which will prevent freezing at -20C. Then, splitting into aliquots is rather pointless and is only required for storage at lower temps. With the 50% glycerol, exposure of the antibodies to freeze/thaw cycles is prevented when stored at -20C, but I still discourage storage in frost-free devices even with the 50% glycerol.

We recommend aliquoting antibodies into working aliquots (~10-20uL or more depending on you usage) and storing the antibodies at -20C. Having one "working aliquot" that your are currently using should be fine to store at 4C short term. There should be no issues, but if fractions do contain glycerol they will not freeze at -20C and long term storage at -70C is recommended. We recommend the working aliquot solution in order to limit the amount of freeze/thaw cycles that Jan spoke about above.