Pretty sure your problem is an MHC mismatch. Do you have any reason to think that C57 cells will cause disease in a mouse where they can't recognize the MHC I or II? You need to use NOD cells for transfer into NOD.scid or C57 cells for transfer into Rag-KO.

You may have to make sure that your NOD-SCID mice (and the animal facility) are free of pinworms or any other infections. I once grew monoclonal antibodies against homing receptors (and control antibodies) and injected them into NOD mice to avoid them getting autoimmune diabetes - but after loosing a year without clear results it became clear the animal house was not as clean as it was supposed to be. Most likely, either the infection with pinworms (or any addtl. infections) or the treatment of the animals by the veterinarian doctor (who had not informed the scientists about treating the animals against pinworms) reduced the ability of NOD mice to get the autoimmune diabetes. So, after injecting mice with antibodies three times a week (and even getting a ticket for "speeding" at 15 mph in a 10 mph zone on a friday night) I was quite happy to focus more on my real and different experiments with no animals. I suggest you ask the responsible veterinary doctors if for any reason there were any infections in the animal facility or the animals were treated with any medications you were not informed.

Is there any way for you to track the cells to make sure the cells have transplanted? CD45.1 vs CD45.2 backgrounds? GFP?

Pretty sure your problem is an MHC mismatch. Do you have any reason to think that C57 cells will cause disease in a mouse where they can't recognize the MHC I or II? You need to use NOD cells for transfer into NOD.scid or C57 cells for transfer into Rag-KO.

In agreement with Oliver Burton, it seems to be an unusual choice of model to use different strains as donor and recipient. It would seem more likely to generate a graft versus host disease model rather than an organ specific colitis model. The B6 and NOD do share 1 MHC class I allele, however, it is not a complete MHC mismatch.

@ Robert Eibl Thanks for the suggestion ..I will check for the infection possibility.

@ Oliver Burton Thanx for the help. I will redesigned everything again. Can you suggest me number of cells need to b injected?

0.5-1x10^6. Are you injecting iv or ip? I think ip may work. But, I've only tried iv as you get better engraftment.

I absolutely agree with Oliver Burton's comment.

Dear Neeraja, you can check this link:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643911/

I agree with Oliver Burton. Nevertheless, I remember that at he facilitiy I worked with NOD mice (and not adoptive transfer), others tried to use adoptive transfer efforts to NOD.Scid mice. As far as I remember -and most likely due to the infection problem- those other experiments did also not have publishable success. Unfortunately, many immunology labs have problems with infections, but your problem might be the choice of genetic backgrounds.

IV through tail vein or retro orbital injections are likely to increase engraftment over IP.

I agree with Oliver and Tamar. I suggest you to read the reference provided by Tamar. C57Bl/6 T cells were used in RAG ko and Balbc - in scid.

We used successfully and with very constant findings a model transfering naive CD4+CD62L+ cells from Balb/c to C.B-17 SCID mice (for testing MSC in IBD treatment). Please refer to our UEGW abstract published in Gut 2010 (59) Suppl. III A302. If any further questions, inquire personally by email.

As with Jochen, we used Balb/C mice CD45RB high sorted T cells (400K/mouse, injected ip) into CB.17 NOD SCID and get disease. In addition, anti-IL-12 treatment inhibits disease development.

@Jochen Maul thanks. @ Tamar Kapanadze thanx for the link.