It takes 3 hours for one sample. Essential oil quantity is less, varies from 0.1 to 0.6%. If seed is old, quantity will reduce. Sample size should be large. Essential oil from coriander is lighter than water and is light pale. Clevenger system can be used.
Further procedure adopted for essential oil profing is given here. After decanting and drying of the oil over anhydrous sodium sulphate the corresponding mild yellowish coloured oil were recovered and calculated in terms of percentage. Whereas Gas Chomatography-Mass Spectroscopy was used for essential oil profiling. One microlitres of volatile oil extracted from cumin seeds was injected to a HP 5 MS column (Agilent, USA, 30m×0.250mm film thickness 0.25 μm) using auto sampler (Agilent, 7693). The analysis was carried out under the following conditions: oven temperature was programmed at 50 ºC for 3 min followed by raising at 10 ºC/min to 180 ºC and 45 C/min to 280 C, injection port temperature was kept 250 ºC; detector temperature 250 ºC; carrier gas: helium; flow rate 1 ml/min, split ratio was 10:1. Authentic standards of major constituents of cumin essential oil were procured from Sigma- Aldrich (USA). These standards were run alone and in combination to get retention time of each constituent. Retention indices of all the constituents were determined by chemstation software (Agilent technologies, USA). The volatile constituents were identified by a comparison of their retention indices and their identification was confirmed by computer matching of their mass spectral fragmentation patterns of compounds in the NIST-MS library and published mass spectra.
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