What are the disadvantages of using knockout mice?

Hello all, I'd like to get further insights on the disadvantages of using knockout mice/global knockout. Also, how can the one further study the effect of this gene/s' knockout. Thanks in...

06 July 2018 5,342 3 View

Which is a better formula : 2^ddCT or 2^dCT in comparing gene expression in 2 different mice strains?

Dear all, I am wondering what is the best way to compare gene expressions in Taqman qPCR. Is it the 2^ddCT values or the 2^dCT? I want to compare the relative expression of different assays in...

03 April 2018 9,945 4 View

To graduate with a PhD or Dr.rer.nat?

02 March 2018 1,951 5 View

Why is the gene expression in TLR4 knock-out mice only reduced but not completely absent when the targeted gene was knocked out in TLR4 KO mice?

When I did the qPCR experiment using the TLR4 assay on spinal cord RNA of TLR4 KO mouse, the TLR4 mRNA was measurable. I did the genotyping of this tissue sample, and it was a homozygous clean KO...

11 December 2017 2,526 3 View

How long a tissue fixation in 4%PFA can last before damaging the SC?

08 September 2016 9,310 10 View

Search engine for proteins and their relevant pathways?

03 April 2016 898 3 View

Any advice on RNA isolation of skin sample for rt-PCR?

Hi, To extract RNA properly out of a whole Skin sample (mouse), how can I homogenize the sample? would the ceramic beads be enough for that? and later, which is better, TriFast or Trizol? Thanks...

10 November 2015 3,438 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View