A simple protocol for plant microsome separation?

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How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What is the reason of stacking DNA in agarose gel well?

we electrophoresis PCR product on 0.7% agarose gel at 120V for 1 hour, our target size is 5000 bp but our DNA stack at wells and do not com in to gelldo you have any suggestion for this?

02 July 2024 5,235 0 View

Is it possible to increase the intensity of UV-vis absorption of DNA EITH A LIGAND IN the groove BINDING mode?

Is it possible to increase the intensity of UV-vis absorption in the interaction of the groove connection mode?

29 June 2024 4,867 4 View

Hello, I wanted to know if it is a necessity to perform MTT test in three time points to evaluate the cytotoxicity of a metal alloy?

I am working on a magnesium based alloy, and i wanted to evaluate the cytotoxicity of my alloy with MTT assay. I want to know the reason why most of the researchers perform the MTT assay in three...

28 June 2024 2,570 1 View

Population coverage analysis for some MHC II alleles?

Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...

20 June 2024 2,155 1 View

Separating Wind Wave and Swell Parameters in Wavewatch III Model?

Hello, I am using the Wavewatch III model for my research . Could anyone clarify if it's possible to extract wind wave and swell parameters separately from this model?

12 June 2024 3,269 4 View

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

10 June 2024 9,523 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How do I western blot of serum proteins without any unspecific binding of secondary antibody to my blot?

Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...

03 June 2024 5,993 8 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Mohamed Abdallah Turky

Good URL for you :-

Minute™ Plant Microsomal Membrane Extraction Kit

http://www.inventbiotech.com/plant-microsomal-membrane-extraction-kit.php

Fatemeh Ranjbar

Thank you Mohmed, It's a good idea, but at first I really like to learn the process like making buffers ,...

I mean, I prefer to learn how to do it in the lab without any Kit.