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17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

What do you think the percentage of educational lost by students in courses where labs and workshops are necessary?

During the current pandemic, most educational institutions all over the world were forced to on-line education which led students to be away from practice applications such as labs and workshops....

30 October 2020 3,254 16 View

What is the relationship (equation) between node transmission range and node density in wsn?

I am simulating routing protocol in wsn the orginal simulation was based on 802.11 mac and physical layer and I am using 802.15.4 as you know the transmission range in 802.15.4 is less, which...

20 August 2020 8,888 2 View

Are you interested in IEEE & Scopus Indexed Conference ?

Dear Colleagues, I am pleased to announce the 3ICT 2020 conference where you can publish your research finding without travelling. All papers registered and presented at 3ICT2020 conference will...

23 July 2020 4,077 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How the annotations of MIT-BIH Arrhythmia Database can be viewed in MATLAB?

Basically i want all classes of arrhythmia in single .mat file with proper labels. What should i do?

09 February 2021 8,062 2 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View

What is the best nanoparticles that can be easily prepared & can be used in silencing the BACE1 gene for prevention of Alzheimer disease?

I want to do a research about the nanotechnology and management of Alzheimer. I found a lot of ideas about nanoparticles that already studied in that field but I do not know which of them is the...

20 January 2021 1,052 3 View