Zuriah Ismail

8 Questions 9 Answers 0 Followers

Questions related from Zuriah Ismail

Do we need to increase volume of transcription enzyme as volume of rna increased too?

I faced some problem in constructing a standard curve for my gene of interest. The GOI is somehow a low copy number genes. Therefore, I'm going to increase the starting amount of cDNA by 500 ng...

24 February 2017 6,972 1 View

Do I still need to isolate pure compound even though the fraction activity is lower than the crude extract using bioassay-guided method?

Hi there, I need some opinion. I've conducted my research project using bioassay- guided method. Out of three sample crude extract I've used, only two of them showed an activity. I've further...

07 December 2016 3,470 8 View

Why luciferase activity decrease when concentration increase?

I was working with luciferase assay to see the ability of my compound as a ligand for receptor. The result somehow does not follow dose dependent manner. it reachs optimum fold change at low...

12 August 2016 4,049 3 View

Does anyone know the detail of silylation method using MSTFA and pyridine?

Hi there.. I would like to seek for an answer regarding the method of silylation using MSTFA and pyridine. As I've go through many journal and protocol, the minimum weight of sample to be...

19 June 2016 9,128 6 View

What is the meaning of negative value percentage of inhibition? The value of absorbance of sample are higher than the absorbance of negative control?

I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...

21 December 2015 1,314 6 View

Can anyone help me in polyhydroxy alkaloids?

I'm currently working with an aquatic plant. I want to extract and isolate polyhydroxy alkaloids from it. My problem is that I'm afraid that I have done a big mistake. I dried the plant and...

01 January 2015 8,688 3 View

How to silylate samples using Sigma Sil A?

I want to do silylation using Sigma Sil A. Anyone know what is the protocol for this derivatization reagent? Where can I perform this procedure (fume hood)? Is it just directly put 100...

06 July 2014 5,675 2 View

I want to isolate polyhydroxy alkaloids using IE-X. What is the best column (size, type and etc.) and the best resins should I used? Thank you

Isolate using ion-exchange chromatography.

25 February 2014 8,668 1 View