Zubaida Marufee Islam

5 Questions 11 Answers 0 Followers

Questions related from Zubaida Marufee Islam

How to make Rotavirus Plaque assay work?

I have been trying to make the Rotavirus plaque assay work. Currently, I am using MA104 cells and RRV strain. I am not being able to see any plaques. I am following the article called "Culturing,...

31 January 2024 3,816 0 View

Can someone suggest me an antibody that binds to microvesicles isolated from cell culture?

I have been isolating microvesicles from HT29 and MA104 cell lines. However, I am not sure which antibody to use to validate the isolation. I have been reading about selectin, integrin and...

30 November 2023 110 4 View

What does the asterisk represent in Jbrowse2 reading frames?

I have a reference sequence from NCBI and it shows the three reading frames. Does the asterisk mean an unknown amino acid or a stop codon?

03 November 2023 141 0 View

Does TopFluor PS analogue gets embedded into the plasma membrane of cell?

Does anyone know if the TopFluor PS (Phosphlatidyserine) analog when incorporated into a cell by liposome-mediated transfection process gets embedded into the actual membrane for visualization?

23 August 2023 7,041 2 View

How can I improve protein concentration after Exosome Isolation from HT-29 cell line?

Hello, I am trying to isolate exosomes from HT-29 cells by PEG precipitation followed by a 100,000Xg Ultracentrifugation wash. I am constantly getting low protein concentrations. I start with a...

27 April 2023 1,804 4 View