Zoya Khan

6 Questions 11 Answers 0 Followers

Questions related from Zoya Khan

Does Bacteria contamination affects whole genome sequencing of cyanobacteria?

Hi, I am working with cyanobacteria and want do Whole genome sequencing of Pseudanabaena (a cyanobacteria). I cannot get axenic culture of it , it is contaminated with other bacteria. Every time...

06 November 2017 1,832 4 View

How to analyse16S-23S ITS region sequences?

I am working with cyanobacteria ITS sequences. Which software should i use to align, to predict secondary structure model and to know the distinct types of ITS? Thank you

28 June 2017 4,353 2 View

Why my DNA got salt pellet?

I did DNA extraction of cyanobacteria. Up until the very end it seemed to be working fine. The Chloroform/isoamly alcohol (24:1) steps all formed a separate layer when introduced and seemed to be...

09 November 2016 7,338 6 View

Pls suggest possible reasons in low mapping of whole genome?

Hi, I have extracted DNA from pure Pseudanabaena (cyanobacteria) culture for whole genome sequencing (illumina Miseq). However, after sequencing it shows very low mapping with its reference genome...

17 May 2016 3,120 8 View

Please suggest protocols for good DNA purity?

Hi, I am doing cyanobacteria DNA extraction. I have used many preassembled kits for DNA extraction but could not get good purity. I tried ethanol purifictaion but i lost 60% of DNA. Pls suggest...

05 April 2016 2,315 5 View

What primers should I use to detect scytonemin biosynthesis gene cluster in Pseudanabaena?

Does anyone knows about scytonemin production in Pseudanabaena?What primers should i use to identify scytonemin biosynthesis gene cluster in Pseudanabaena or any other close relative of it?

27 May 2015 9,445 1 View