Zixun Han

5 Questions 16 Answers 0 Followers

Questions related from Zixun Han

Why the two protein solution become cloudy after mixing?

I have two protein X and Y, X protein isoelectric point is ~5.5, Y is ~9.5. western blot result indicated that they can directly bind each other. I want to purify large amount of this XY complex...

10 October 2016 7,428 8 View

All the proteins are inclusion bodies, how to purify soluble protein?

i aim to purify the soluble human PCID2 protein (1-399aa) for crystallization. I have constructed several different plasmid, like GST-tagged pGEX-PCID2 full length, His-tagged pET28a-PCID2 full...

06 June 2016 7,839 12 View

How can i degas without a degas machine?

our degas machine was broken today, and it will be repaired at next month. but recently i will use FPLC to purify protein, so how can i degas the buffer, milli-Q, and 20% EtOH?

06 June 2016 2,673 5 View

Does "different origins of replication" is essential in co-expression?

recently i learn some knowledge about co-expression. the EMBL website said, in the case of co-expression from different vectors, To ensure plasmid stability, the vectors should have:(1)different...

06 June 2016 9,536 4 View

How can I remove bound RNA of RNA binding protein during purification?

I have a problem for several months. I desperately need your help. I have two tagged proteins GST-A and His-B, both of them are RNA binding protein, pI of GST-A is 6, pI of His-B is 9. Previous...

09 September 2015 8,032 5 View