Zimeng Ye

4 Questions 13 Answers 0 Followers

Questions related from Zimeng Ye

How to determine if two genetic mutations are in cis or in trans?

Hi all, Does any one know how to determine if two different genetic mutations in the same gene are on the same allele (cis) or on the opposite allele (trans)? They are in the same gene but are...

14 March 2019 6,412 6 View

Did anybody use ddPCR to detect methylation?

We have 9 candidate methylation markers of interest, and we want to target at them. It is not advisable to use illumina 450k beadchip, because it only covers one marker of interest. I have seen...

05 February 2018 7,117 2 View

MGB probes vs. LNA probes, which one is better for allelic discrimination?

Hi all, When you are using ddPCR to detect singe point mutations (eg: T>G), and the DNA concentrations of the samples are very low, which probe do you think is better? MGB or LNA? Many thanks!

18 January 2018 3,654 4 View

Does anybody know the DNA concentration of cerebrospinal fluid in normal control people?

Hi all, I am trying to compare the DNA concentration of CSF in cancer patients and normal controls with ddPCR. Does anyone know about the average read of copies in normal controls? Thanks ahead.

15 January 2018 6,076 3 View