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Can I use a synthesised RNA fragment containing the amplification target to construct a standard curve for a one-step RT-PCR? Can I also use the RNA fragment as the amplification control? What...
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I am designing a one-tube nested real-time PCR. Only one probe will be used and the signal will be only collected from the second stage. Any suggestions for primer and probe TM design?
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As mentioned in the title, has someone compared the running speeds between SYBR Green-DNA polymerase - amplicon and conventional polymerase -amplicon in an agrose gel?
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I have tried this method (http://2012.igem.org/Team:Cambridge/Protocols/TransformationofB.subtilis) as well as electroporation, but I cannot get the transformant. The labs in our building do...
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