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Questions related from Zhengrong Zhou
Recently I bought 100 mg proteinase K power from Roche (3115879001, Proteinase K,recomb.,PCR Grd.l), and then I add 5 ml into the bottle to make the concentration reach 20 mg/ml. However, I found...
21 July 2015 8,691 7 View
Hello everyone! When I search the protocol of whole mount in situ hybridization, I found the time often from E8.5 to E11.5 or E12.5. Whether the time point of whole mount in situ hybridization...
16 May 2015 3,830 6 View
I want to transfect the hippocampal neuron by lentivirus (with GFP), so I produced the lentivirus myself and the virus was resuspend in DPBS(maybe mix some FBS from the media) after...
22 April 2015 6,066 5 View
In the culture of neuron, the serum is avoided in the media. Does it just inhibit the growth of astrocytes? Or the serum in the neuron culture media would cause any other problems? What is the...
01 April 2015 1,337 7 View
I am looking for the protocol of RIPA buffer in the internet and found many different kinds of RIPA buffer, some have NaF and some without, does anyone know the function of NaF in the RIPA...
13 March 2015 5,747 8 View
I used Glutathione Sepharose 4B to purify a protein fused GST tag, and the protein was in a dialysis buffer (20 mM Tris-HCl, 100 mM NaCl, pH 7.4). However, after dialysis overnight, some white...
09 September 2014 8,747 4 View
In cell culture, we often use polylysine (PLL)-coated glass coverslips to culture cells. The PLL can improve cell adherences. Does anybody know how the theory of PLL improves cell adherences?
07 May 2014 7,071 3 View
