Zhang Xin

3 Questions 4 Answers 0 Followers

Questions related from Zhang Xin

Are there other ways to deliver inositol 1,4,5-trisphosphate (IP3) to the cytosol than caged IP3?

Hi all, To activate the IP3 receptor on ER and trigger the calcium signaling, the IP3 has to be delivered to the site without any pre-activation. I learned that the established method is to use...

16 March 2018 3,669 2 View

What could cause such huge difference of calcium influx in cells?

Recently I am repeating my mitochondrial calcium measurements with the protein sensor 4mt TNXL which is a FRET sensor working like the CFP/YFP-pair sensors (4mt D3cpv) but with a higher Kd. But...

12 September 2017 3,809 3 View

How to calibrate the FRET signal and get the correction factors with Zeiss Axiovision?

Recently we upgraded our Zeiss cell observer D1 for the real time imaging experiment with ratiometric probes and FRET probes. And for the calculation of the "real" FRET signal, the Axiovision...

12 September 2017 6,013 2 View