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Questions related from Zeynep ZÇ Çeli̇k
Hello, I'm having trouble isolating MIA PaCa-2 cells. No bands form on the agarose gel. I've tried numerous times, but the results haven't changed. I've tried the same protocols on PANC-1 without...
5 weeks ago 244 1 View
While the GAPDH ct value in 2D Panc1 cells is 21, the GAPDH ct value in Panc1 spheroids is 13. Is this result correct? If not, what could be the reason for such a result? I would be very grateful...
last month 8,618 2 View
CDKN2A gene expression in pancreatic cancer cells is between Ct 36-38 at 50 and 100ng (Enzyme Activation 95oC, Denaturation 95oC, Annealing/Extension 60oC). What could be the reason?
08 August 2025 4,667 1 View
Why did the pellet not dissolve during manual rNA isolation in MCF-7 Spheroids?
11 July 2025 5,549 3 View
MCF-7 Sferoidlerinde manuel rNA izolasyonunda pellet neden çözünmedi? Pellet çözünmediği için RNA miktarı düşük geldi ve jelde bant oluşmadı.
10 July 2025 3,636 4 View
