Zexu Chen

4 Questions 4 Answers 0 Followers

Questions related from Zexu Chen

Poor status of cultured iPSC: contamination or differentiation?

My iPSC clones in mTeSR plus culture had bad performance recently. There seem to be excessive dead cells and clumps sticking to the clones. The dead cells can not be removed by flapping the dish....

21 February 2023 3,006 2 View

Is the rescue experiment necessary in the knock-out model?

I have generated a CRISPR/Cas9 knock-out zebrafish of my desired gene (referred to abc-/-) and an overexpression line of abc by using Tol2 transposon (referred to Tg(hsp7:abc)). All the phenotype...

19 May 2022 2,676 1 View

Are my IPS clones dying?

I recovered the IPS line three days ago according to a standard protocol, however, there seems to be necrosis in the clones. Things get worse today. I use the mTeSR plus and change the spent media...

05 October 2021 437 4 View

How to test the specificity of a home-made antibody in in the lack of mutants when WB works badly but IF works well??

I have a home-made affinity-purified antiboy. It works badly in WB but turn out to be great in IF. But we should test the specificity of the staining. However we don't find any mutant of this gene...

06 September 2019 9,603 4 View