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Questions related from Zexu Chen
My iPSC clones in mTeSR plus culture had bad performance recently. There seem to be excessive dead cells and clumps sticking to the clones. The dead cells can not be removed by flapping the dish....
21 February 2023 3,001 2 View
I have generated a CRISPR/Cas9 knock-out zebrafish of my desired gene (referred to abc-/-) and an overexpression line of abc by using Tol2 transposon (referred to Tg(hsp7:abc)). All the phenotype...
19 May 2022 2,667 1 View
I recovered the IPS line three days ago according to a standard protocol, however, there seems to be necrosis in the clones. Things get worse today. I use the mTeSR plus and change the spent media...
05 October 2021 425 4 View