Zankruti Dave

10 Questions 15 Answers 0 Followers

Questions related from Zankruti Dave

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

23 February 2021 1,642 6 View

What causes low 260/230 ratios?

Hi, I have been doing RNA isolation using the standard RNAeasy Plus mini kit. The 260/280 ratios seem fine - between 1.8 - 2-0, however the 260/230 ratios have been consistently low ( below 1.. as...

12 May 2020 7,924 3 View

Is it possible to filter antibody solutions ?

Hi, I have this antibody against the protein of my interest which was a gift from another lab so I do not have the possibility of getting it again. The commercial antibodies are not as good as...

30 August 2018 6,471 0 View

Why does my plasmid concentration seem to be increasing over time?

Hi, I have a set of plasmids ( midi-preps isolated using a standard kit). When I had freshly isolated them, I had resuspended the DNA pellet in TE buffer and I have been using these plasmids for...

19 September 2017 4,310 5 View

How to avoid antibody heavy chain interference ?

Hi, So I do a lot of co-IP's and my bait protein has a molecular weight of 56kDa and its a real problem because the antibody heavy chains always seem to interfere. I have tried using clean blot...

11 August 2017 9,428 10 View

Minipreps and Maxipreps keep failing.

Hi , For the past 2 weeks I have been struggling with something so simple. I have a plasmid ( vector is pcDNA4/TO with amp resistance). It has worked perfectly before and recently I was close to...

27 February 2017 9,551 5 View

How to solve Endogenous IP problems ?

Hi, I need help understanding what could be the issue with my endogenous Co-IP's. The TCL shows I have enough amounts of the proteins but my IP samples hardly show any signals and most of the...

13 February 2017 9,336 8 View

Does anyone have a good suggestion for a good ROR1 Antibody?

Hi everybody, I am looking for a good antibody to detect endogenous levels as well as over-expressed levels of ROR1. So far, in our lab, we were using an antibody that was gifted by another lab...

25 January 2016 5,837 4 View

Any suggestions for a good pan SFK inhibitor?

Hi, i am working with a few members of the src family (Lyn, fyn,blk) and was wondering if anyone can suggest a good inhibitor which can target the src family. Most of the ones on market are...

25 June 2015 3,139 3 View

What is the best way to extract cells from a tumor sample?

I need to extract live cells from ovarian tumors. I have tried this only twice so far and have not been successful. Once I get the tumor sample, usually immersed in PBS, I remove it in a petri...

01 November 2014 4,641 9 View