Zainab Ghrefa

3 Questions 9 Answers 0 Followers

Questions related from Zainab Ghrefa

How can we increase the quantity of exosomal RNA for qRT-PCR?

Hi, I am using a miRCURY RNA Isolation Kit to extract exosomal RNA from 0. 5 ml serum and I got very low volume. Then I reduced the elution volume ( from 100 µL to 50 µL) in order to make it more...

01 January 2015 9,770 4 View

Can anybody help me to understand what is the problem with these membranes ?

western blot for exosomes sample. I used antiGAPDH and antipalladin antibodies. I load 20ug of sample, in 1xRIPA buffer and 4xLaemmli buffer. Images 4&6 same membrane.

11 November 2014 4,138 8 View

Is it possible to isolate HDL-cholesterol by using iodixanol without other lipoproteins (LDL, VLDL)?

I need a clear protocol in order to isolate HDL only from blood by using optiprep (iodixanol). Is it possible to isolate HDL without other lipoproteins(LDL,VLDL) and without staining? Because I...

05 May 2014 3,936 3 View