Zahra Damirchi

4 Questions 3 Answers 0 Followers

Questions related from Zahra Damirchi

Should I use from "WHO International Standard Somatropin (Recombinant DNA-Derived Human Growth Hormone) NIBSC 98/574" as standard in SDS-PAGE?

1) Hi, Do you know that NIBS 98/574 standard is used in SDS-PAGE? (in this standard COA, it's not mentioned that the above standard is suitable for SDS-PAGE or not?) 2) why my recombinant human...

12 December 2019 8,882 5 View

How do you calculate your results in Bradford protein assay method?

I've used from Bio-Rad Protein Assay kit (Bradford technique), I measure my micro-plate absorbance in 595/450 nm ratio and I report the ratio blanked OD.Is it correct? And I usually see some...

10 October 2019 3,025 5 View

What is the best Antibody for detecting Human Growth Hormone with western blotting technique?

Hello, I am looking for a suitable antibody for hGH. I wanna to confirm my result with a confirmatory test such as Western Blot. Thank you.

07 July 2019 8,099 3 View

Why the main band of hGH doesn't match with 22 kDa band of MW marker in SDS-PAGE?

Hello, I use from hand-cast polyacrylamide gel 14% and Bio-rad Precision Plus Dual Color Standard as MW Marker.My standard band and main band of my samples (somatropin) often are below 20 kDa and...

06 June 2019 664 4 View