Zahra Rashidbenam

4 Questions 5 Answers 0 Followers

Questions related from Zahra Rashidbenam

Which concentration of Rapigest should be used for protein extraction from a tissue sample?

I am trying to extract protein from a tissue sample for liquid chromatography mass spectrometry but I am not sure which concentration of Rapigest to use (0.1 %, 0.5% or 10%). Has anyone prepard...

29 May 2018 8,192 4 View

Which epithelial cell line can be used as positive control for urothelial cell marker expression in vitro?

I am trying to find a suitable epithelial cell line to be used as positive control for the markers Uroplakin Ia, Uroplakin II, cytokeratin 7 and Cytokeratin 20. The one currently reported in...

19 January 2018 5,629 2 View

What Is the best protocol for isolating Smooth muscle and Urothelial cells from bladder biopsy?

I need to isolate smooth muscle cells and urothelial cells separately from bladder biopsy. What will be the best method do that?

18 May 2017 393 2 View

Is it possible to store tissue (Bladder) for a while before isolating its cells?

I need to isolate smooth muscle cells and urothelail cells from bladder biopsy. I am wondering if it is possible to store collected tissue sample for a while before processing without affecting...

18 May 2017 6,145 3 View