Zack Saud

5 Questions 9 Answers 0 Followers

Questions related from Zack Saud

Is it suboptimal to place a viral solution contained in glass or plastic in a sonication bath in comparison to probe sonication (at same frequency)?

We have a sonication bath, but I was just wondering if purchasing a probe sonicator would lead to better disaggregation of viral particles for both purification and electron microscopy purposes,...

01 January 2018 6,223 1 View

Does anyone have any suggestions of how to remove Aluminium ions from a sample of extracted DNA?

I have extracted DNA from a vaccine containing aluminium hydroxide gel using sodium citrate (tribasic). The extraction has work well and I have a good qubit reading for dsDNA as well as a good...

06 June 2017 3,950 2 View

What is the optimum copy number of starting DNA template for PCR?

I understand the reasons why too much starting template can inhibit the formation of PCR amplicons, and I am aware of the guideline amounts of DNA in weight/volume measurements. But obviously the...

05 May 2017 2,311 6 View

I'm trying to extract DNA from a poxvirus in a vaccine that contains aluminum hydroxide gel. Can anyone suggest a technique to purify the virus?

I am trying to extract DNA from a poxvirus in a vaccine containing aluminum hydroxide gel. I wish to purify the virus by removing it from the aluminum hydroxide gel matrix, as the gel, or a...

03 March 2017 4,594 2 View

Anyone know of a good online resource which allows automatic annotation of whole-exosome sequencing data (ie flags up unhealthy genes in data)?

Websites similar to www.promethese.com but for Illumina Hiseq NGS data.

02 February 2016 5,150 7 View