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I am quantifying the molar ratio of hapten to cbsa using maldi tof ms for my conjugation work. unfortunately,cbsa doesnt give any signal using maldi tof ms.but,tnbs shows the positive result...
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what is the best method to reduce sample loss during purification process?I did column by using silica gel to remove unwanted compound. but, the yield of the compound is about 30% of the starting...
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