Why would gel electrophoresis need to be run for 16 hours at 2-8 deg C? Is there an alternative protocol that takes less time or works at room temp?

I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...

19 November 2020 752 2 View