Yusri Dwi Heryanto

2 Questions 3 Answers 0 Followers

Questions related from Yusri Dwi Heryanto

Can we use E.G7-OVA cells for negative control in a study to test the specificity of probe targeting CD20?

We want to test specificity of our probe for targeting CD20 but I don't have Jurkat cells or MOLT-4 cells for negative control. I have E.G7-OVA cell(mouse T cell lymphoma). Can we use E.G7-OVA as...

27 March 2015 3,303 4 View

Is there anyone who has experience conjugating IRDye 700 with oligonucleotide?Can you give me the protocol?

I incubate IRdye700 with antisense oligonucleotide phosphorotioate for about 12 hours in Na2HPO4 buffer. (MW IRDye700 = 1954; MW Oligonucleotide = 5844 ) When I purify the result using Aktaprime...

16 March 2015 5,103 3 View