Yunuo Wang

6 Questions 12 Answers 0 Followers

Questions related from Yunuo Wang

I have one drug Urelumab and need to binding the urelumab with BV421 color ,how should I do that?

I want to staining Urelumab ,but urelumab itself has no color,if I want to staining it with colors like BV421 ,how should I do?binding them with another antibody?

01 January 2016 687 2 View

What's the difference of anti human antibody and anti mouse antibody ?

I want to stain the human TIL with cell marker antibody.When I try to order the antibody..I notice that some of the antibodies named anti-human antibody and some named anti-mouse antibody..It's...

05 May 2015 579 5 View

How many PBMC per well should I have when I want to stimulate T cell with CD3/CD28 ?

How many PBMC per well should I have when I want to stimulate T cell with CD3/CD28 beads? I will only run them on Flow. In this experiment I want to see the expression of the three surface marker...

03 March 2015 1,009 7 View

How can I avoid too much cell on the chart edges with flow cytometry?

I am the beginner of the flow cytometry . When I analyze my data of healthy PBMC I found out more than 60% of my cell is on the chart edges.What cause this phenomenon..and how to solve this..I...

03 March 2015 5,484 8 View

Why PD-1 expression go higher if I do nothing to them only to put them outside on the medium for 24 or 48 hr?

Why PD-1 expression go higher if I do nothing to them only to put them outside on the medium for 24 or 48 hr? I think it should be stimulate ..then the PD-1 will express higher

03 March 2015 8,304 4 View

How many cells will be ok for CD3/CD28 stimulated flow?

I am very new to flow. I stimulated the Healthy PBMC and want to get the stimulate T cell, but I don't know if I should keep the same cell numbers in all wells? So far, I set unstained 250,000...

02 February 2015 760 3 View