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Questions related from Yunlong Hu
I was asked about the concept and difference between PCR & sequencing and PCR-direct sequencing, I was confused about it. Is there someone who can help me? Thanks a lot!
09 September 2013 1,291 3 View
Previously, vector transcription was always alright. Recently, after transcription, there is no target RNA. I don't know the reason, different vectors and new reagents like sp6, RNAse, 25mMdNTP,...
05 May 2013 6,131 1 View
Changing the protein solution from 8M urea to 2M urea with dialysis, existing protocols suggest 8M to 6M then 4M and last to 2M, what's the reason? Can we change the solution directly from 8M to...
03 March 2013 3,734 11 View
What's the immunogenic characteristics of an insoluble protein, such as a membrane protein? Can we use the precipitated insoluble protein for the immunization of a rabbit, what's the result if...
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