Yuming Wang

6 Questions 10 Answers 0 Followers

Questions related from Yuming Wang

Can anyone help with the introduction of two mutations in one gene by CRISPR?

Hi Everyone. I want to use CRISPR-Cas9 system to knock in two mutations of one gene in cells, but I would like these two mutations occur on different alleles, i.e. one allele carries one mutation...

07 July 2015 4,235 5 View

Is it possible to make site directed mutagenesis in plasmids larger than 15 kb?

Hi All, I want to generate a mutation in plasmid, but my plasmid is around 16 kb, is it possible to do it? I am planning to design two non-overlapping primers containing the mutation site to PCR...

07 July 2015 3,531 3 View

Can anyone suggest a differentiation protocol for SK-N-SH?

Hi All, I have SK-N-SH cells I would like to differentiate, what protocol do you normally use? I can see that people do it in different ways, I want to know which method gives you the most...

10 October 2014 3,934 1 View

Does anyone have experience on HOPC cells?

my question is very simple: I've purchased some human oligodendrocyte precursor cells from Sciencellonline, when I received the cells, I read something quite disappointing on the datasheet: do not...

08 August 2014 7,940 3 View

How do you extract good quality of RNA from frozen brain tissues?

Hi all, I had a bad experience on extracting total RNA from frozen human brain tissues. The PMI for all tissues is from 3 to 22 hours. I used the Qiagen RNeasy Lipid kit and tissueruptor to...

08 August 2014 3,331 3 View

Does anyone have problems recovering neuroblastoma cells from ATCC?

Hi all, Recently I got a frozen vial of SH-SY5Y cells from ATCC, I've been recovering them in a small T25 flask for two weeks, but they don't expand at all and look quite stressed. After I...

08 August 2014 1,682 20 View