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I'm inducing M0 macrophages from THP-1 by using Phorbol 12-myristate 13-acetate (PMA) But frome time to time, I encounter induction failure (twice now), which could be fixed by new PMA stock. I...
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Hi, I'm culturing THP-1 and induce them into macrophage. And I run into some issues: I culture 2x10^5 cells / ml and seeding 2x10^6 cellsin a 6 well plate, treat 20ng / ml PMA for inducing them...
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Hi, I'm currently seeding 293T in 6 well plate (5*10E5 / well) for transfection. However, the condition of cells seems unable to adhere and growth well, this happened 24hrs after seeding, not...
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I am polarizing macrophage from THP-1 cells, then treat macrophage with conditional medium. But the gene expression is so unstable that I can’t properly examine the problems. The experiment method...
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Hi, I'm performing MTT assay recently and found out the purple color seems to fade through time. The color would fade from like fig.1 to fig.2 (schematic, different plate) in around 3 days, no...
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