Yuhung Zheng

6 Questions 1 Answers 0 Followers

Questions related from Yuhung Zheng

Problems with PMA for induce M0 from THP-1?

I'm inducing M0 macrophages from THP-1 by using Phorbol 12-myristate 13-acetate (PMA) But frome time to time, I encounter induction failure (twice now), which could be fixed by new PMA stock. I...

06 March 2022 8,919 1 View

Questions on culturing THP-1 cells, any suggestions?

Hi, I'm culturing THP-1 and induce them into macrophage. And I run into some issues: I culture 2x10^5 cells / ml and seeding 2x10^6 cellsin a 6 well plate, treat 20ng / ml PMA for inducing them...

15 February 2022 2,106 3 View

Does anyone have experience on RNA immunopercipitation (RIP)?

Hello, I’m performing RIP experiment recently and I run into some issues. Below is simplified version of my protocol 1. collect cell (I’m using 1/2 of a 6 well plate of 293T) 2. Pellet cells, add...

02 January 2022 2,966 1 View

293T died after seeding, can anyone help?

Hi, I'm currently seeding 293T in 6 well plate (5*10E5 / well) for transfection. However, the condition of cells seems unable to adhere and growth well, this happened 24hrs after seeding, not...

01 January 1970 457 2 View

Macrophage Polarization is unstable

I am polarizing macrophage from THP-1 cells, then treat macrophage with conditional medium. But the gene expression is so unstable that I can’t properly examine the problems. The experiment method...

01 January 1970 325 1 View

Does MTT assay color faded through time?

Hi, I'm performing MTT assay recently and found out the purple color seems to fade through time. The color would fade from like fig.1 to fig.2 (schematic, different plate) in around 3 days, no...

01 January 1970 1,506 2 View