Yuganthini Vijayanathan

2 Questions 5 Answers 0 Followers

Questions related from Yuganthini Vijayanathan

How to resolve a consistent NTC band near target size of a Phusion reaction?

Hi all, I am getting a consistent band near the target size (1.4kb) at the NTC of a Phusion reaction. I have changed all my stock reagents: Phusion buffer, Phusion enzyme, dNTP, Nuclease-free...

28 June 2022 462 3 View

Why does samples on poly-l-lysine slides keep on falling of though there were no heat treatment done on it?

For immunohistochemistry tissue samples are normally mounted on slides coated with poly-l-lysine. But these paraformaldehyde fixed tissues keeps falling of newly coated slides. The thickness of...

13 March 2017 9,981 3 View