Yue Lu

3 Questions 7 Answers 0 Followers

Questions related from Yue Lu

Did anyone ever experience the shrinkage of muscle frozen section after fixation by Bouin's solution?

I'm recently working on the Gomori's trichrome staining on adult mouse limb. The whole lower limbs (skinned) were fixed in 4% PFA at 4C , decalcified in EDTA, embeded in OCT and cut as 20um...

23 September 2016 8,557 5 View

Does vector mouse to mouse IgG blocking reagent works with invitrogen Alexa fluor conjugated secondary antibodies?

Hi, I'm recently working on immunostaining for Pax7 in mouse muscle cryosections. The primary antibody is mouse IgG1 anti-Pax7 (DSHB) and it seems the only antibody working in mouse with a Mouse...

19 May 2015 9,263 2 View

Does anyone have a protocol for tendon cryosection?

I'm recently working on mouse tendon cryosection for immunostaining. But when I cut these tendon samples, they scattered so it's very hard to get intact sections. I've tried to changed the...

14 April 2015 5,756 6 View