Yuan Zhang

6 Questions 9 Answers 0 Followers

Questions related from Yuan Zhang

How do indel mutations affect transcription?

I have some crispr mutants that have indel mutations, mostly 1bp insertion or deletion. Most 1bp indel resulted in pre-mature stop codons during translation. However, I don't know whether the mRNA...

25 November 2018 5,552 6 View

What is the difference between series dialysis of 8M urea and one-time dialysis?

I tried 8M Urea buffer for plant protein extraction and nickel purification. I equilibrated the nickel beads with TEV cleavage buffer which contains 50mM tris, phosphate, 300mM NaCl. Then I added...

21 March 2017 5,392 6 View

How many proteins in a rice cell?

I noticed a drastic expression difference for the same protein between Arabidopsis and rice. I am wondering why. Though I used equal amount plant material for immunoblotting, my protein abundance...

10 January 2017 7,063 3 View

Inconsistent transgene expression among transgenic lines under a constitutive promoter in plants?

I transformed a 6His tag fusion gene driven by AtUBQ10 promoter and expressed it in Arabidopsis. In T1 transgenic plants, first I used genotying to confirm they were real transgenic plants; In...

08 December 2016 5,375 7 View

Why my transgene does not express as high in T4 generation as early generation in Arabidopsis??

I just obtained homozygous line of one of my overexpression lines. I chose this line for propagation because its expression was highest among other transgenic plants when I use western blotting to...

03 November 2016 9,651 4 View

Up or down-regulated genes in crispr mutants

I would like to know whether I can use RT-PCR to check mRNA level of possible downstream genes in the crispr mutants of Arabidopsis. The mutation type of the genes in these crispr mutants are...

01 January 1970 849 1 View