What is the effect of sodium pyruvate in DMEM when differentiating 3T3-L1 adipocytes? Can I differentiate them in DMEM with pyruvate for tranfection?

I have a protocol for reverse transfecting 3T3-L1 adipocytes, which differentiates them in DMEM without pyruvate, 25 mM glucose, 10% fetal calf serum (FCS), and 1% penicillin and streptomycin...

20 January 2023 6,410 0 View