Yoojin Seo

4 Questions 2 Answers 0 Followers

Questions related from Yoojin Seo

What`s wrong with my Th1 / Th2 induction protocol?

Hi all, I tried to induce th1/2 polarization with human naive CD4 T cells with protocol below. 1. isolate the naive CD4 T cells from commercially provided human PBMC (with MACS) 2. Seed isolated...

02 March 2017 6,304 0 View

Can neural stem cell give rise to Purkinje neurons?

Hi, I`ve found that ES or iPSC can be differentiated into Purkinje neurons.However, I cannot find any publication dealing with NSC conversion to Purkinje neurons. Since I do not have much...

11 December 2015 6,846 3 View

Can anyone explain the differences between ATP cytotoxicity and NLRP3 inflammasome activation?

Hi, everyone. I`m trying to activate NLRP3 inflammasome in various cell types with classical method (LPS + ATP). While I reviewed this process, I noticed that the concentration of ATP I`m using...

17 February 2015 6,636 4 View

Can anyone help with LC3 expression and Rapamycin /Bafilomycin treatment?

Hi all, I started studying autophagy and found it is very complicated mechanism. I got some concepts about autophagy as followed: -when autophagic process is activated, LC3 I is converted to LC3...

09 January 2015 9,657 6 View