Yonghong Ding

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Questions related from Yonghong Ding

How is PFBHA quenched by concentrated sulfuric acid?

In my experiment, PFBHA is used to derivatize a carbonyl group containing compound and then the excessive PFBHA is quenched by added concentrated sulfric acid according to previous studies. I...

04 April 2015 6,653 1 View

In BN-PAGE, why do we need to replace the heavy blue cathode buffer with light blue cathode buffer, after the dye running to one-third of the gel?

I cannot find any detailed explanation regarding this question, please help! In blue native PAGE, almost all protocols mentioned to replace the heavy blue cathode buffer (i.e. in my case, 0.02%...

11 November 2014 3,702 4 View