Yong Jia

6 Questions 23 Answers 0 Followers

Questions related from Yong Jia

How to interpret the results from PSI-BLAST?

Hi, I have been trying to use the PSI-BLAST (NCBI) standalone tool to do a comprehensive homolog search in a self-generated fasta database. In my case, the blast search iterates 4 times before it...

06 June 2016 4,689 5 View

Why does the absorbency at 340nm decrease without enzyme and substrate, NAD degradation at basic condition?

Hi, I am having a problem while doing enzyme assay. At 100mM Tris-HCl, ph9.0, in my control reaction containing just NAD+ without enzyme and substrate, the absorbency at 340nm...

06 June 2015 1,343 25 View

Why does the NAD+ 340nm absorbance increase without the addition of substrate during enzyme assay?

I got another weird observation with my enzyme assay. The control reaction containing just NAD+ and my enzyme sample without the addition of substrate displays consistent signal increase at 340nm....

06 June 2015 3,372 7 View

Why Autodock tools could not find the VF.py or subprocess.py ?

I got a problem while using Autodocktools, when it comes to Run-->Autogrid or Autodock, there is always an error as the attached figure shows. I tried both window and linux versions, both of...

04 April 2015 1,693 11 View

How can I compute Bayesian posterior probability for a given phylogenetic tree?

After a search for a suitable phylogenetic tree using RAxML, how can I compute the Bayesian posterior probability for the resulted tree. What programs shall I use? Thanks.

01 January 2015 1,256 12 View

How long may enzyme activity be preserved in storage at 4 degree?

Hi, I am about to carry out a few enzyme assays. After protein purification and dialysis to remove the salts, what is the acceptable maximum time to keep the protein at 4 degree before performing...

11 November 2014 2,954 11 View