Yong Du

5 Questions 7 Answers 0 Followers

Questions related from Yong Du

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

I was expressing a protein in Ecoli BL21 and did the following purification steps: 1- GST purification (Pierce™ Glutathione Agarose 16100) 2- GST tag cleavage (Presscission protease: Genscript...

06 October 2020 693 7 View

Is there any cre mice can avoid fetal death?

I am looking for a cre mice that mediates gene removal in the late embryo phase or after birth. The embryo with total knock out may died on E12. Anyone can help?

25 August 2017 1,925 0 View

How to avoid "mice grind food" when doing food intake study?

Recently, I was doing a food intake study. I single-caged mice and measured food consuming each day. Some mice grind food into powder, which causes an inaccurate meaurement. I put a wooden block...

18 January 2017 1,575 3 View

How to explain my TG mice has a high glucose and leptin level at 3 month young age, but with no difference in insulin level?

why glucose increases, but insulin doesn't change?

01 July 2016 4,885 6 View

How to explain a gene's highly expressed in tumor and correlates with metastasis and poor surviral but not highly expressed in metastatic tumor?

I found a oncogene's expression was higher expressed in tumor more than normal both in tissues and cell lines. Besides, we also found its expression correlates with patients T and M phase and poor...

20 March 2016 9,325 8 View