How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

I was expressing a protein in Ecoli BL21 and did the following purification steps: 1- GST purification (Pierce™ Glutathione Agarose 16100) 2- GST tag cleavage (Presscission protease: Genscript...

06 October 2020 586 7 View