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Hi everyone, I have a big trouble when doing EMSA. I used the biotin-labeled probe to detect protein-RNA complex. however, the whole well path is so smear that I cannot find a clear single band....
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Hi everyone, I am trying to detect some microRNAs by Northern Blot using biotin-label probe. I used trizol to isolate total RNA. However, I cannot detect microRNAs of interest. Do you have any...
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Anyone has experience to add a NLS to the protein of interest?
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