Ying Chen

5 Questions 3 Answers 0 Followers

Questions related from Ying Chen

Anyone is expert in cell death?

hi all, I am new in doing cell death. when I treat cells with TNFa and Birinapant at different timepoint, I observed that more cell float up with longer treatment, but when I did Western Blot to...

11 June 2020 8,372 0 View

The bands are smear for EMSA?

Hi everyone, I have a big trouble when doing EMSA. I used the biotin-labeled probe to detect protein-RNA complex. however, the whole well path is so smear that I cannot find a clear single band....

16 November 2018 9,163 4 View

How to enrich the micrRNA using trizol-based method?

Hi everyone, I am trying to detect some microRNAs by Northern Blot using biotin-label probe. I used trizol to isolate total RNA. However, I cannot detect microRNAs of interest. Do you have any...

26 October 2018 6,363 4 View

How to add a nuclear localization signal to protein of interest?

Anyone has experience to add a NLS to the protein of interest?

17 August 2018 1,073 5 View

QPCR for mature microRNA?

hi, everyone. I am detecting mature microRNA expression level these days using taqman probe in vivo system. I want to know whether I can still trust data when the Cq value of microRNAs reach 30?

09 November 2017 7,010 2 View