Yeping Sun

5 Questions 8 Answers 0 Followers

Questions related from Yeping Sun

How get a protein excrete from HEK 293F cells?

Dear everyone, I am trying to express the extracellular domain of a membrane protein in HEK 293F cells. The construct contains the signal peptide of the protein itself. However, the protein does...

26 November 2021 3,913 7 View

How to remove the metal ion from a metal protein?

Dear all, We are studying a protein which contains a coordinated bivalent cation (Mn2+/Fe2+/Zn2+). My first questions is how to determine whether the metal ion is present in the protein we...

02 November 2020 457 3 View

How to download the complete natural products library of mol2 format from ZINC 15 database?

I wish to download the natural products from ZINC database (https://zinc.docking.org) with wget command: wget 'https://zinc.docking.org/substances/subsets/natural-products.mol2?count=all' but...

24 March 2020 4,173 5 View

How to analyze cryo-EM reconstruction map and prepare publishable figures?

Dear Professors, I am new to cryo-EM structural biology. Now I have a cryo-EM reconstruction map for a virus particle. I wish to analyze the characteristics of the virus, such as the h, k and t...

11 September 2019 9,663 4 View

How to define trisymmetrons and pentasymmetrons on the capsid in chimera?

Hello everyone, I have study the structure of icosahedral virus caspid. I know it can bee divided intotrisymmetrons and pentasymmetrons, but I find it is difficult to define trisymmetrons and...

01 January 1970 6,698 3 View