Yasuhiro Endo

3 Questions 2 Answers 0 Followers

Questions related from Yasuhiro Endo

What is the most appropriate media for TNFa-treated HUVECs ?

I am Yasuhiro Endo , a medical doctor in Japan. I am going to incubate HUVEC with 10 ng/ml tumor necrosis factor-α (TNF-α; R&D systems, Abingdon, UK) or PBS as control for 5hrs and measure...

07 December 2021 4,115 0 View

Would you tell me how to dissolve cholesterol in RPMI medium?

We are planning to add cholesterol to cells in order to raise the intracellular cholesterol concentration and confirm the protein expression of SREBP2. I didn't know if I should dissolve...

15 January 2021 1,000 6 View

Could you tell me how to inject fluids via tail vein of C57BL6 mouse ?

I am a graduate student in molecular biology in Japan. In the experiment, I am in trouble because I cannot administer the drug from the tail vein of the mouse.Can you tell me how to train mice for...

16 October 2020 2,913 0 View