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I am Yasuhiro Endo , a medical doctor in Japan. I am going to incubate HUVEC with 10 ng/ml tumor necrosis factor-α (TNF-α; R&D systems, Abingdon, UK) or PBS as control for 5hrs and measure...
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We are planning to add cholesterol to cells in order to raise the intracellular cholesterol concentration and confirm the protein expression of SREBP2. I didn't know if I should dissolve...
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