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Questions related from Yasu Xu
I want to use two promoters to express two gRNA in a single plasmid and wish them to have same number(i.e. two promoter with nearly same strength). I am not sure whether I should insert a...
06 June 2019 7,000 1 View
I want to redo a RNA extraction from the E.coli cells and I store the liquid culture (in TB) for two days. I am not sure whether I can still use them or I need to regrow some cells.
02 February 2019 7,906 2 View
I was expecting only ~110bps amplicon in my sample, but I see a very broad band. Attached is the result. 2 and 4 only contain the template, no primers. Also, I think my intensity is kind of low.
11 November 2018 6,803 0 View
I want to compare transcription of a gene both inserted in the genome and a plasmid. Should I extract the RNA in the exponential phase or stationary phase?
11 November 2018 2,050 11 View
I want to count the amount of crRNA expressed in a plasmid with Ecoli. I am not sure whether I can use a RT-qPCR to do it. The length is between 50 - 100bps.
09 September 2018 1,149 6 View
As above
06 June 2018 6,750 0 View
