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I use the 1 ml of water extract of dried Spirulina (1gm/5ml),added to 25 ml of 1 mM AgNO3 then left in light shaking to get a purple solution instead of the brown one of the AgNPs.
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why do i get a high o.d for ascorbic acid in concentration of 5mg/ml in dpph assay is this concentration is v. high for calibration curve setting up?
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