Yassin Elfaki

3 Questions 4 Answers 0 Followers

Questions related from Yassin Elfaki

Is it possible to compare the diversity and the degree of overlap of TCR repertoire in different WT mice (non-TCR tg)?

Dear colleagues, We are attempting to compare the TCR repertoire (diversity and degree of overlap) of different T cell subsets among different non-TCR tg mice by using NGS. Is it feasible...

07 July 2018 7,098 0 View

Can an ELISA assay for glucocorticoids (GCs) measurements be used in assaying GCs in tissue lysates?

I would like to measure GCs in tissue samples and I have tissue lysates. Is it feasible to use an ELISA-based assay or is there a better alternative? Thanks in advance!

04 April 2018 8,252 1 View

When staining ex vivo cells for FACS analysis, can I fix the cells and stain them for transcription factors (esp. T-bet) the next day?

I am staining cells for FACS for cytokines and transcription factors. Is it possible that I postpone the intracellular staining one day without compromising quality of satining?

06 June 2016 4,178 3 View