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I want to differentiate between Tobacco mosaic virus and Tomato mosaic virus without sequancing. Note: I mechanically inoculated tomato ( Solanum lycopersicum) but no symptoms appear.
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I want to prepare a buffer containing 0.5 g of MgCl but I found the bottle of MgCl.6H2O totally hydrated and become liquid . So can I use it ? And how to take this weight as volume ? Thank you...
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i want to test the specificity of degenerate primers for a virus NCBI does not support degenerate primers so if any one know how can i solve this or how to use De-MetaST-BLAST tool ? Thank you ...
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i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...
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